Determination of Centromere Number of Chromosomes Involved in Robertsonian Translocation by using Fluorescence in Situ Hybridization (FISH)

Tulin Cora 1 * , Hasan Acar 1, Yusuf Özkul 2
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1 Selcuk University, Selcuklu Medical School, Department of Medical Genetics, Konya
2 Erciyes University, Medical School, Department of Medical Genetics, Kayseri
* Corresponding Author
EUR J BASIC MED SCI, Volume 2, Issue 1, pp. 1-5.
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Robertsonian translocation is one of the major chromosomal re-arrangements and constitutes 18% of all genetic abnormalities with an incidence of 1/1000 in the general population. Robertsonian translocations are characterized centric fusion of the long arms of the acrocentric chromosomes and generally occur between chromosome 13q, 14q and 14q,21q. In this study, the centromere numbers of 22 patients with Robertsonian translocations were evaluated by using fluorescence in situ hybridization with repetitive DNA probes localized on the centromeric region of acrocentric chromosomes. We detected that all Robertsonian translocation have a single cetromere. However some of case showed a distinct size of FISH signals on cetromere region, which may indicate the duplication or two different satellite DNA sequences. Therefore these cases should be clarified for the composition of monocenric centromere sequence.


Cora T, Acar H, Özkul Y. Determination of Centromere Number of Chromosomes Involved in Robertsonian Translocation by using Fluorescence in Situ Hybridization (FISH). Eur J Basic Med Sci. 2012;2(1):1-5.


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