The Effects of Melatonin and Endothelin-A Receptor Antagonist BQ-123 in Exposed to Smoke in Rat Renal Tissue

Hasan Erdogan 1 * , Fatih Ekici 2, Huseyin Aslan 3, Birsen Ozyurt 4, Filiz Erdogan 5, Omer Atis 6
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1 Namık Kemal University, Faculty of Medicine, Department of Physiology, Tekirdag
2 Yıldırım Beyazıt University, Faculty of Medicine, Department of Physiology, Ankara
3 Gaziosmanpasa University, Faculty of Medicine, Department of Histology and Embryology, Tokat
4 Gaziosmanpasa University, Faculty of Medicine, Department of Anatomy, Tokat
5 Tekirdag State Hospital, Department of Obstetrics and Gynecology, Tekirdag
6 Ataturk University, Faculty of Medicine, Department of Medical Biology, Erzurum
* Corresponding Author
EUR J BASIC MED SCI, Volume 3, Issue 1, pp. 1-8.
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It has been known that smoking increases the level of Endothelin (ET), a very powerful vasoconstrictor agent in circulation, causes damage and functional impairment of tissues. In our study, it was aimed to evaluate the effects of melatonin, a potent antioxidant, and BQ123 on renal tissue of rats exposed to cigarette smoke. Wistar albino 34 adult male rats were divided into four groups; Control, Smoking, Smoking+Melatonin, and Smoking+BQ-123 groups. They were exposed to cigarette smoke in the vacuum-operated glass cabinet 3 times a day, 30 minutes for 28 days. Melatonin was administered i.p. 25 mg/kg/every day. BQ-123 was injected from tail vein i.v. 1 mg/kg, on the first, 7th, 14th, 21th, 27th day. After the rats were sacrificed on the 28th day, the biochemical parameters were analyzed with spectrophotometric methods and tubular cell accounts for stereological analysis were done by optical fractionator technique. Renal tissue SOD activity was significantly increased in the Smoking+Melatonin group according to the control and Smoking+BQ-123 groups. Catalase activity was significantly decreased in the Smoking+Melatonin according to the other groups. Lipid peroxidation level was significantly increased in Smoking group in comparison with the Control and Smoking+BQ-123 groups. Protein Carbonyl and Nitric Oxide levels, and GSH-Px activity have not statically significant among the groups. Tubular cell accounts were significantly increased in the given of Melatonin and BQ-123 groups according to the Control and Smoking groups. We concluded that cellular damage generated by smoke in renal tissue of rats partially reduced by Melatonin and BQ-123 depending on the dose and duration with different pathways. This conclusion was supported by histologic staining and stereologic analysis with increase of the cell number of renal tissue in rats.


Erdogan H, Ekici F, Aslan H, Ozyurt B, Erdogan F, Atis O. The Effects of Melatonin and Endothelin-A Receptor Antagonist BQ-123 in Exposed to Smoke in Rat Renal Tissue. Eur J Basic Med Sci. 2013;3(1):1-8.


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