Development of Rapid Diagnostic Reagents for Respiratory Tract Infections in Children

Kei Numazaki 1 *
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1 Division of International Infectious Diseases, Graduate School and Departments of Pediatrics, University Hospital, International University of Health and Welfare
* Corresponding Author
EUR J BASIC MED SCI, Volume 5, Issue 4, pp. 51-60.
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Rapid diagnosis of respiratory tract infections is important not only from the standpoint of clinical diagnosis but also infection control. The purpose of the present study is to investigate the possibility of diagnosing with the immunochromatography for pathogens of respiratory tract. A prospective, populationbased study covering the total population less than 15 years of age in Tochigi, Japan, during the period of December 2010 to March 2013. Nasopharyngeal swabs were collected from 200 children with respiratory tract infections. RTPCR analysis was used as the reference assay. The correlation of the results between obtained by the diagnostic reagents and RT-PCR assays was investigated. Pathogens were identified in a total of 64 (32.0%) out of 200 patients by RT-PCR as follows: respiratory syncytial (RS) virus, 29; M. pneumoniae, 17; metapneumovirus, 7; adenovirus, 5; influenza A virus, 3; and influenza B virus, 3. More than 100 copies/μl of Mycoplasma pneumoniae was obtained. Minimum detection sensitivity of the immuno-chromatography in the clinical material was the 4 x 101copies/micro-liter. The correlation of the results between obtained by the diagnostic reagent for measles and RT-PCR was the positive match rate of 45.5% and negative match rate is 100%. From 79.0 to 100% of RT-PCR positive samples more than 4 x 101copies/micro-liter was detected. The importance of M. pneumoniae and RS virus in the etiology of respiratory tract infections in was confirmed. Immuno-chromatography can be applied as a simple rapid diagnostic method from the beginning of the disease in general clinical practice.


Numazaki K. Development of Rapid Diagnostic Reagents for Respiratory Tract Infections in Children. Eur J Basic Med Sci. 2015;5(4):51-60.


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